Fig. 8. Transduction of acini with Ad constructs. (A,B) Phase microscopy images of untransduced and Ad-LacZ-transduced acini, respectively, processed for detection of ß-galactosidase activity with X-Gal as substrate. (C,D) Fluorescence microscopy images of untransduced and Ad-GFP-transduced acini, respectively. (E,F) Confocal fluorescence microscopy images of untransduced and Ad-Dynt-transduced acini, respectively, processed for detection of dynamitin by immunofluorescence. (G) The spectrum of ³⁵S-labeled proteins obtained by autoradiography of proteins from control and transduced acini resolved by SDS-PAGE. Arrows indicate positions of ß-galactosidase (ß-Gal), GFP and dynamitin (p50). H shows dynamitin content in lysates from untransduced (Con), Ad-Dynt-transduced and Ad-Dynt-GFP transduced acini by western blotting. Transduction with constructs was at a MOI of 5 PFU/cell for 4 hours followed by rinsing and recovery for 16-18 hours. Bars in D and F represent 10 µm and reflect magnifications in A-D and E-F, respectively. Asterisk, lumenal regions.

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