Fig. 6. Effects of unc-60A (RNAi) on polar body extrusion, cortical activity and anterior-posterior polarity. The unc-60A (RNAi) treatment resulted in the appearance of an extra pronucleus (a, arrow) owing to the lack of polar body extrusion and formation of abnormal membrane protrusions (b, arrowheads). (c-e) Measurements of markers for the anterior-posterior polarity in the one-cell embryo. (c) The position of pronuclear meeting was measured as x/Lx100, where x is the distance from the anterior pole (A) to the site of pronuclear meeting and L is the length of the egg between the anterior and posterior (P) poles. (d) Similarly, the positions of the anterior spindle pole (y/Lx100) and the posterior spindle pole (z/Lx100) were measured. Bars, 20 µm. (e) Data from control or unc-60A (RNAi) embryos (n=17) are presented by a box plot generated by SigmaPlot 2000 (SPSS Science). Boxes indicate the ranges between 25^th and 75^th percentiles, and bars on both sides indicate ranges between 10^th and 90^th percentiles. Bars in the boxes indicate medians.

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