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Fig. 8. Characterization of the unc-60B-null mutants. (A) Exon-intron structure of the unc-60 gene (McKim et al., 1994) and the locations of deletions in unc-60 (s1586) and unc-60 (su158). (B) Motility of wild-type, unc-60 (su158), or unc-60 (e677) homozygous adults. Data are means±s.d. n=10. (C) Expression of UNC-60A and UNC-60B in unc-60B mutants. Total lysates (10 µg proteins) from wildtype (lane 1), unc-60 (su158) (lane 2) and unc-60 (e677) (lane 3) were resolved by SDS-PAGE and visualized by Coomassie blue (a) or subjected to western blot with anti-actin (b), anti-UNC-60A (c) or anti-UNC-60B (d). The strong loss-of-function mutant unc-60 (e677) has a greatly reduced amount of UNC-60B (d, lane 3), whereas the unc-60 (su158) mutant has no detectable UNC-60B (d, lane 2). Molecular mass markers in kDa (lane M) are indicated on the left of a.





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