Fig. 9. Assembly of myofibrillar components in the unc-60B-null mutant embryos. Wild-type (a-c) or unc-60 (su158) (d-f) embryos at the 1.75-fold stage (430 minutes after first cleavage) (A), two-fold stage (450 minutes) (B) and three-fold stage (520 minutes) (C) were stained with anti-actin (a,d), anti-myoA (b,e) and anti-vinculin (c,f) antibodies. Actin and myoA were stained by double-labeling of the same embryo. In Bd and Cd, arrows indicate the regions where actin is markedly disorganized. The age of the embryos was determined from their morphology as described previously (Epstein et al., 1993; Hresko et al., 1994). Bar, 20 µm.

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