Fig. 1. Lysates from bait L40 yeast strains carrying plasmids encoding the indicated fusion proteins were analyzed for protein expression by immunoblot. LexA fusion proteins were detected using anti-LexA or anti-N-RAP antibodies, as indicated. The results show that the bait strain expressed an intact LexA-N-RAP fusion migrating at 170 kDa, whereas unfused LexA protein migrated at 26 kDa on SDS-PAGE. Also shown are results from a LexA-lamin fusion construct migrating at 35 kDa and used as a negative control for N-RAP binding experiments.

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