Fig. 3. (A) Schematic diagram showing the domain organization of N-RAP (top), along with the regions of N-RAP expressed as histidine-tagged fusion proteins. Numbers refer to amino acid residues from the full-length mouse N-RAP open reading frame (Luo et al., 1997). (B) -actinin binding to N-RAP recombinant proteins immobilized on Ni-NTA agarose beads were analyzed by SDS-PAGE. (C) Duplicate loadings were blotted and probed with -actinin antibody. The antibody specifically detects -actinin binding to N-RAP-IB and N-RAP-LIM, but does not detect any -actinin binding to N-RAP-SR or to the His-CAT control proteins.

Return to article