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Fig. 4. (A,B) Filamin binding to blotted proteins. Duplicate loadings were used (A)
for total protein detection with Coomassie blue and (B) for immunoblot
detection of bound filamin. Lanes were loaded with purified proteins as
indicated. The histidine-tagged CAT proteins (HIS-CAT-1, HIS-CAT-2) served as
negative controls. Significant filamin binding to recombinant N-RAP-SR was
observed. (C,D) Filamin binding to N-RAP recombinant proteins immobilized on
Ni-NTA agarose beads. Duplicate loadings were used (C) for total protein
detection with Coomassie blue and (D) for detection of bound filamin.
Significant filamin binding to recombinant N-RAP-SR was observed.
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