Fig. 6. AKAP149-derived RVXF peptides induce intranuclear lamina disassembly in G1 phase. (A) RVXF or RAXF peptides (10 nM) were injected into G1- or S-phase nuclei, as indicated, and 30 minutes later cells were fixed and analyzed by double immunofluorescence using anti-B-type lamin and anti-lamin A/C antibodies, and using anti-LBR and anti-nucleoporin (mAb414-reactive; Nups) antibodies. The injection solution contained phenol red (Ph, red). Between 45 and 55 cells were injected/treatment. Non-injected (input) G1 cells were also labeled. Bars, 10 µm. (B) Cells were metabolically labeled for 1.5 hours with ³²P between 0.5 and 2 hours after release from mitotic arrest (G1) or while under mitotic block (M). B- or A-type lamins were immunoprecipitated and IPs analyzed by autoradiography and immunoblotting. A, B and C point to lamin A, B-type lamins and lamin C, respectively.

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