Fig. 6. Archvillin in hamster skeletal muscle is primarily sarcolemmal, localizing at costameres and within peripheral nuclei. The -H340 signal localizes predominantly as `arches' in optical cross-sections (B,D, double arrowheads) that resolve as alternating circumferential thick and thin bands with occasional longitudinal striations in confocal sections within the plane of the sarcolemma (C, inset, arrow). Archvillin colocalizes with costameric dystrophin in both transverse (D-F) and oblique (G-I) muscle sections. Colocalization with ethidium homodimer-1 in peripheral nuclei also is seen (J-L, arrows), as is a small amount of staining within the muscle cells (M-O, asterisks). Phase (A), epifluorescence (B) and confocal fluorescence sections (C-O) show the colocalization of archvillin (B,C,D,G,J,M) with anti-dystrophin (E,H) and ethidium homodimer-1 (K,N). Composite images (F,I,L,O) were generated by superimposition of the -H340 signals in green and dystrophin (E,H) and ethidium homodimer-1 (K,N) signals in red; areas of overlap appear yellow or orange. Bars, 5 µm (C), 10 µm (A-B,G-I) and 20 µm (D-F,J-O).

Return to article