Fig. 8. EGFP-tagged full-length murine archvillin (MAV-FL) colocalizes with endogenous archvillin, F-actin and non-muscle myosin II. Confocal fluorescence images showing C2C12 myoblasts visualized for (A) endogenous archvillin localization with -H340 in nuclei (arrowhead) and at the plasma membrane (arrow); (B,C) colocalization of EGFP and -H340 signals in cells expressing low (arrows) and moderate (arrowhead) levels of EGFP-tagged archvillin; and colocalization of (D,G) EGFP-tagged full-length archvillin with (E) Alexa^TM-594 phalloidin-stained F-actin or (H) antibodies against non-muscle myosin II heavy chain. Composite images (F, I) were generated by superimposition of the -H340 signals in green and phalloidin (E) or nonmuscle myosin II (H) signals in red; areas of overlap appear yellow. Myoblasts in panels G-I were pre-extracted with 0.2% Triton X-100 (5 minutes, 0°C) to better visualize cytoskeletal structures. Bar, 5 µm.

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