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Fig. 9. Increased amounts and polarization of endogenous and EGFP-tagged murine archvillin in myotubes. Confocal images showing increased amounts of archvillin staining in untransfected myotubes (A,B), as compared with adjacent myocytes (asterisks). In addition to the overall increase in staining, large clusters of fluorescence are observed for endogenous archvillin (B) and for EGFP (C,E) and {alpha}-H340 signals (D,F) at the tips of myotubes expressing low levels of EGFP-tagged murine archvillin (EGFP-MAV). In both small (C,D) and large (E,F) myotubes, EGFP and {alpha}-H340 archvillin signals colocalize at myotube tips (arrows) and as punctae along the membrane and within the cell. Bars, 20 µm.





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