Fig. 6. Effects of BFA treatment on the localization of cPLA₂-. Cells were stimulated with 5 µM A23187 (in HEPES/Tyrode's buffer) in the presence of 1 mM extracellular Ca²⁺ for 1 minute. BFA (10 µg/ml) was then added to the cells for 30 minutes and the cells were fixed and permeabilized. cPLA₂- was then detected by immunofluorescence microscopy. Bar, 10 µm.

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