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Fig. 6. Recovery of ROP2 expression restores rhoptry shape, mitochondrial
association and virulence in mice. After prolonged passage of ROP2AS
clones in VERO cells, ROP2 protein expression recovered to nearly wild-type
levels (A). A random sample of 31 vacuoles from `recovered' ROP2AS-7
clones was examined by transmission electron microscopy. Parasite vacuoles and
individual organisms appeared more normal (B,C). An increasing number of
vacuoles are associated with host mitochondria (B,C), as confirmed by
morphometric analysis (see Fig.
3E). Most rhoptries are restored to the original club shape with
an electron dense distal portion (D,E). Survival curves of mice infected with
recovered ROP2AS clones are similar to those for wild-type parasites
(Fig. 6F). Wild type (
); ROP2AS-1 (
); ROP2AS-7
(
); ROP2AS-20 (
). Bar, 1.0 µM (B-C), 0.2 µM (D), 0.4
µM (E).
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