Fig. 4. SkpA^– larval CNS cells accumulate supernumerary centrosomes. (A-D) DNA and centrosome staining in wildtype and skpA^– cells. (A-B) SkpA^– interphase cells 3.5 days AED have abnormally condensed chromatin (arrowhead) compared to wildtype. DNA that labeled with P-histone H3 antibodies is marked (M). (C) skpA^– metaphase cell with four centrosomes labeled with antibodies against -tubulin and centrosomin (cnn). (D) Polyploid (16C) skpA^– nucleus from larva 5.5 days AED that partially labeled with P-histone H3 antibodies and contained 24 centrosomes. Bars, 5 µm. (E) Quantification of centrosomes in mitotic wildtype and skpA^– cells. The distribution of P-histone H3-positive cells with different numbers of centrosomes is shown. (F) Quantification of polyploidy in mitotic wildtype and skpA^– cells. Polyploid P-histone H3-positive cells were identified from measurements of their total DNA content. Error bar represents one standard error. (G) Electron micrographs of skpA^– cell from 5.5 days AED larval CNS with four pairs of centrioles in nine 105 nm sections. A low magnification section with two pairs of centrioles is shown, with high magnification views of centrioles from this or adjacent sections shown in the insets. Bars, 500 nm.

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