Fig. 5. Supernumerary centrosomes in skpA^– cells nucleate microtubules. (A-F) Projections of confocal sections of mitotic skpA^– neuroblasts (A-E) or ganglion mother cell (F) labeled for DNA (P-histone H3 or TOTO-3), centrosomes (cnn) and microtubules (-tubulin). Cells have the following number of centrosomes: (A) 4, prometaphase; (B) 10, metaphase; (C) 12, metaphase; (D) 8, multipolar prometaphase; (E), 4 and 6, anaphase; and (F) 6, metaphase. Note the two chromosomes pulled off the metaphase plate by supernumerary centrosomes (arrowheads, B), and the spindle pole not associated with any centrosomes (arrow, E). Bar, 2 µm. (G) Quantification of centrosome clustering during mitotic stages. Supernumerary centrosomes in P-histone H3-positive cells from 3.5-day AED larvae were scored as clustered if they were within 2 µm of another centrosome. Mitotic stages were determined from chromosome and spindle morphology. Error bar represents one standard error. The average number and range (solid line) of centrosomes per cell is also shown for each stage. (H) Ratios of metaphase to anaphase cells from larvae of different ages.

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