Fig. 5. DIMZ cell migration rate mediated by 4 constructs. The travel rate (A) and the radial displacement rate (B) were measured using the Nanotrack function within the ISEE software package, then plotted as bar graphs. The 4 constructs expressed and substrates used are as indicated. For each construct, 40 to 65 cells from three different batches of embryos were measured. Error bars indicate standard deviations. Normal DIMZ cells cannot adhere to V-GST, thus their travel rate and radial displacement are not available. 4KV and 4RR cells migrate significantly slower than 4 chimeras, whereas 45 cells migrate the slowest compared with other 4 chimeras. The statistical significance among different 4 constructs is summarized in Table 2.

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