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Fig. 6. DMZ explant migration assay. (A) A DMZ explant. The bottom layer is the mesoderm tissue sheet (red), which encounters the substrate, and the top layer is ectoderm tissue (blue). The dorsal lip edge is positioned up and the arrow indicates the direction of mesoderm tissue sheet migration. (B) The morphology of DMZ explants expressing GFP or {alpha}4{alpha}5, shown as a representative example. The construct injected and the adhesive substrate are as indicated. Arrows point to the migrating sheet of mesodermal tissue. (C) Migration rate of DMZ explants. The distance of leading edge cell advancement in 1 hour is measured and plotted as a bar graph. The substrate and construct injected are as indicated.





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