Fig. 7. Only some cytoplasmic domains are able to support FN fibrillogenesis. (A) FN fibril assembly rescue assay. 4 Constructs were injected into the animal pole regions of embryos at the two-cell stage. At stage 9, mAb P8D4 were injected into the blastocoel of these embryos. The embryos were cultured until the end of gastrulation. Then animal caps were dissected and subjected to immunostaining for FN. (B-J) Immunofluorescence of FN on the blastocoel roof. The 4 construct expressed is indicated on each panel. Endogenous FN fibril network is formed on the blastocoel roof at the end of gastrulation (B). P8D4 blocked fibril formation (C). In the presence of P8D4, 4 chimeras with the 5 and 6 cytoplasmic domains were able to mediate the assembly of significant amount of FN fibrils (H,I). A few fibrils were also observed on the BCR injected with 43 (G). The mAb 4H2 was used to immunostain FN.

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