Fig. 10. Disruption of motors alters the distribution of peripherin. PC12 cells grown on locator coverslips in DM for 48 hours were microinjected with kinesin heavy chain antibody (C,D) or as a control, with non-immune serum (A,B), and then processed for immunofluorescence using peripherin antibody at 0.5-4 hours post-injection. Control cells displayed normal peripherin networks (A). In cells injected with kinesin antibody the peripherin was almost exclusively located in the cell body (C). B and D are phase images of the same injected cells.PC12 cells were also transfected with myc-dynamitin cDNA (G) or mock transfected (E) and processed for immunofluorescence with peripherin and c-myc (data not shown) antibodies, 48 hours post-transfection. Dynamitin-expressing cells showed peripherin staining almost exclusively in the peripheral regions of the cell body and distal regions of neurites (G). Mock-transfected cells displayed peripherin networks that were typical of well-differentiated cells. Phase contrast (F,H). Bars, 10 µm.

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