Fig. 5. Microsomes and cytosol prepared from add37, add39, add66 and add67, but neither add06 nor add68 are proficient for pF degradation in vitro. (A) Microsomes containing pF were prepared from each ADD deletion strain and incubated in the (a) absence or (b) presence of cytosol made from the same strain; the amount of pF was determined at 0 and 40 minutes after addition of cytosol or buffer. (B,C) Microsomes and cytosol were prepared from both WT and deletion strains (B, add06 and C, add68) and analyzed for ERAD in vitro in the indicated combinations of microsomes/cytosol: WT/WT; • WT/buffer; add/buffer; add/add; WT/add; add/WT. The amount of pF was determined using the Bio-Rad Phosphor Analyses program. Data represents the mean of triplicate experiments, ±s.d.

Return to article