Fig. 8. kar2-1 add37 double mutants display a synthetic growth defect. Exponentially growing cultures of dissected tetrads were plated at tenfold dilutions on complete media and incubated at 37^oC for two days. Progeny from crosses add37 X kar2-1 (A) and add67 X kar2-1 (B) were ordered in rows from top to bottom: WT strain, ADD/KAR2; ADD deletion mutant strain, add/KAR2; temperature-sensitive kar2-1 strain, ADD/kar2-1; and the double mutant, add/kar2-1. The results for crosses add06 X kar2-1 and add39 X kar2-1 were similar to that seen for add67 X kar2-1.

Return to article