Fig. 7. Centromere and telomere analysis of Ndj1-HA-expressing haploid control and kar3 SK1 strains after transfer to sporulation medium (SPM). The frequencies (based on >100 nuclei per time point) of nuclei with one centromere FISH cluster in meiotic time courses of haploid sir3 spo13 control [cen cluster (1n control)] and haploid kar3 sir3 spo13 [cen cluster (1n kar3)] drop at similar rates after the induction of meiosis. In the 1n kar3 time course, cen-cluster dissolution stalled between 180 and 240 minutes. All values were normalized with the values at 0 minutes (58% in WT; 56% in kar3) set to 100%. The frequency of bouquet nuclei with one peripheral Ndj1-HA IF telomere cluster peaks at 300 minutes in the haploid control [Ndj1-HA bouquets (1n control)] and kar3 [Ndj1-HA bouquets (1n kar3)] SK1 strain. Ndj1-HA bouquet nuclei are more abundant at all time points in kar3 meiosis. The bouquet frequencies shown are given as the percentage of haploid Ndj1-HA-expressing (meiotic) cells (74% in the WT; 48% in kar3 mutant; Fig. 8). It appears that bouquet nuclei accumulate in absence of Kar3p.

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