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Fig. 8. The frequency of nuclei exhibiting Ndj1-HA fluorescence in meiotic time courses of haploid sir3{Delta} spo11{Delta} control (Ndj1-HA+ hapl. control) and kar3{Delta} sir3{Delta} spo11{Delta} (Ndj1-HA+ hapl. kar3{Delta}) SK1 strains. In the haploid control meiosis, fewer Ndj1p-HA-expressing cells (Ndj1p-HA+) are present at early time points, whereas most cells rapidly enter meiosis after 180 minutes in sporulation medium (SPM). A high frequency of kar3{Delta} cells expressing Ndj1-HA are seen before 180 minutes in SPM, whereas the increase of Ndj1-HA+ cells occurs only gradually over the entire time course. Similar observations are seen when the expression of Zip1p is monitored by IF and all cells displaying Zip1p signals of any nature were scored as Zip1p+. As in the Ndj1-HA study, induction of meiosis in the kar3{Delta} strain failed to induce a significant increase in Zip1p-expressing cells. The Ndj1-HA+ cells at 0 minutes probably result from ectopic sporulation of kar3{Delta} meiocytes.





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