Fig. 6. WIP binding to actin is essential for PDGF-induced ruffle formation. (A) GST pull-down assay. The indicated GST-WIP constructs (5 µg per 10 µl beads) were incubated with 0.05 µM G-actin. After SDS-PAGE, samples were blotted with anti-actin monoclonal antibody. (B) Fluorescence micrographs of TRITC-phalloidin-stained Swiss 3T3 fibroblasts transfected with control plasmid (3T3pcDNA), WIP coding sequence (3T3pcDNA-WIP) or the deletion mutant WIP43-54 (3T3pcDNA-WIP43-54), before and after PDGF challenge. Cells were grown on glass coverslips, serum starved for 3 days in the presence of 0.5% FBS, stimulated or not with PDGF (50 ng ml^-1) for 8 minutes and then fixed and labeled with TRITC-phalloidin to visualize actin filaments. Magnification 400x.

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