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Fig. 1. Polo kinase and stabilized Cyclin A modify the PIMdba phenotype. (A-F) DNA staining at stage 14 reveals the presence of many large polyploid abnormal nuclei in the CNS of embryos expressing the stabilized securin PIMdba if they are also polo mutant (E,F; pimdba polo-). By contrast, at this stage, cells in the CNS are hardly affected in polo mutants that do not express PIMdba (C,D; polo-) or in the polo+ siblings that express PIMdba (A,B; pimdba). B, D and F show high-magnification views with the CNS from the embryos displayed in A, C and E, respectively. (G-J) Using prd-GAL4 and UAS-CycA{Delta}1-53, stabilized Cyclin A was expressed in alternating embryonic segments. Expressing segments are indicated by arrowheads in G and I or by white lines in H and J, which display high-magnification views of epidermal regions. DNA staining indicates that the metaphase delay caused by stabilized Cyclin A is prolonged in embryos that also express the stabilized securin PIMdba under the control of the pim+ regulatory region. Compared with embryos without PIMdba (G,H; CycA{Delta}N), metaphase plates (arrows) in regions with stabilized Cyclin A are enriched in embryos that also express PIMdba (I,J; pimdba CycA{Delta}N).





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