Fig. 4. Effect of EphB4-Fc and ephrinB2-Fc on lateral 2D migration of HUVECs (A) and VEGF-stimulated HUVEC chemotaxis (B). (A) EphB4-Fc (1 µg/ml) and VEGF (50 ng/ml) stimulate lateral EC migration (48 hours). In turn, ephrinB2-Fc (1 µg/ml) inhibits baseline as well as VEGF-induced lateral EC migration (mean±s.d.; analysis of four independent experiments performed in triplicates). (B) EphrinB2-Fc (2 µg/ml), but not EphB4-Fc (2 µg/ml), significantly inhibits VEGF- (20 ng/ml) mediated chemotaxis (4 hours) of HUVECs in a modified Boyden chamber assay (mean±s.d.; analysis of four independent experiments performed in triplicates). Control A: medium, upper and lower compartment; control B: medium, upper compartment, VEGF, lower compartment. **P<0.01 compared with baseline control; P<0.05 compared with VEGF induction.

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