Fig. 5. Overexpression of SFRP1 in chick retinal progenitors increases the number of RGC and cone photoreceptors while decreases the number of amacrine cells. Frontal cryostat sections from retinas of E6 embryos infected with RCAS-SFRP1 viruses at stage 10. Adjacent sections from (A,C,E,G,I,K) infected and (B,D,F,H,J,L) contralateral uninfected central retinas were immunostained with antibodies against the (A,B) Gag viral protein, (C,D) Tuj1, (K,L) the mitotic marker PH3 and the cell-specific differentiation markers (E,F) islet1 (retinal ganglion cells), (G,H) visinin (cone photoreceptors) and (I,J) CRABP1 (amacrine cells). Note how the number of (C,E) cones and migrating (arrowheads) and (A) layered ganglion cells is increased in Gag-positive retinas, while amacrine cells are reduced, without apparent variations in the number of PH3-positive cells. Note also the thickness of the Tuj1-positive fibre layer in (C) the infected retina compared with the thickness of the fibre layer in (D) controls. GC/AM, ganglion cell/amacrine cells; FL, fibre layer; PE, pigment epithelium. Bar, 38 µm.

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