Fig. 10. Mitotic chromosomes of transfected Xenopus A6 cells expressing full-length ZLAP2- GFP (A,B,D) or ZLAP2-GFP (C,E) analyzed by light (A) and electron microscopy (B-E). (A,B,D) The same cell at metaphase is shown by fluorescence microscopy (A) and on electron micrographs of ultrathin sections through this cell (B,D). The metaphase chromosomes are clearly visible at low magnification (B). (D) Higher magnification of the boxed area in (B) shows that chromosomes are associated with vesicles. (C,E) Electron micrographs of an ultrathin section through a transfected cell at metaphase expressing ZLAP2-GFP. The chromosomes are shown in an overview (C) and higher magnification (E, central region of C). A membrane stack (arrowheads in C and E) containing ZLAP2-GFP, chromosomes (single arrows) and spindle microtubules (double arrows) are marked. Metaphase chromosomes of cells expressing ZLAP2-GFP are not associated with vesicles. Bars, 0.5 µm (D,E), 2 µm (C) and 5 µm (A,B).

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