Fig. 5. Distribution of LAP2 during the cell cycle in zebrafish blastomeres. Localization of ZLAP2 in whole-mount preparations of embryos by indirect immunofluorescence microscopy with polyclonal antibodies specific for ZLAP2 (A-F; A'-F', DNA staining by Hoechst). Embryos at the cleavage period (A,B; 1.5 hpf), early blastula stage (C,D; 2.5 hpf) and late blastula stage (E, F; 4 hpf) have been analyzed. Cells at metaphase (A-A',E-E'), anaphase (B-B',F-F'), the karyomere stage (C-C') and interphase (D-D') are shown. (D) The nuclear envelope of the interphase nucleus (D) is highly invaginated, resulting in an apparent intranuclear staining with LAP2 antibodies. This morphology is characteristic for nuclei that have been formed by the fusion of karyomeres. Digital images were taken with a CLSM (C-C',D-D',E-E',F) and a Zeiss Axiophot (A-A',B-B',F'). Bars, 10 µm.

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