Fig. 4. Xfz3 dimerization is sensitive to ß-mercaptoethanol. Protein extracts from embryos overexpressing Xfz3C-myc were analyzed by western blot with the anti-myc antibody. Before SDS-PAGE, protein samples were incubated with 2% or 20% ß-mercaptoethanol. This treatment leads to a diminution of the dimer/monomer ratio. The dimeric form of the receptor is still strongly detected in extracts treated with 10 mM iodoacetamide (IAA), which prevents the artificial formation of disulfide bonds during sample preparation.

Return to article