Fig. 6. Dimeric forms of Xfz7 are not detected after overexpression in Xenopus embryo. (A) Direct immunoblot analysis using anti-myc antibody of protein extracts from gastrula embryos overexpressing Xfz7-myc, Xfz3C-myc or Xfz3-myc. In protein extracts derived from Xfz7-myc-injected embryos, the anti-myc antibody revealed one group of three major bands corresponding to the expected molecular weight for Xfz7-myc; no band at the size of the putative dimeric form is clearly detected. By contrast, monomeric and dimeric forms of Xfz3-myc and Xfz3C-myc are observed. (B) Embryos were injected with mRNA encoding Xfz7-myc or Xfz7C-flag, or both. Protein extracts from injected embryos were subjected to immunoprecipitation by using the anti-myc antibody (lanes 1 and 5) or the anti-flag antibody (lanes 2, 3 and 4). Immunoprecipitates were then immunoblotted with the anti-myc antibody (left panel) or anti-flag antibody (right panel). Anti-flag antibody does not immunoprecipitate any myc-positive bands from embryos expressing both Xfz7-myc and Xfz7C-flag.

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