Fig. 10. Model depicting interactions of Sla1p and Sla2p at the cell cortex. (1) Sla2p associates at the cell cortex with clathrin light chain and also potentially directly with the plasma membrane through its ENTH domain. (2) The central region of Sla2p is able to recruit Sla1p (through an interaction with a region encompassed by residues 118-361 of Sla1p) and the associated endocytic machinery Pan1p/End3p. (3) Sla1p also binds to the actin-associated proteins Abp1p and Las17p/Bee1p, allowing recruitment of actin patches to these endocytic sites These interactions then lead to the reorganisation of the actin cytoskeleton, which is likely to promote endocytosis. Evidence for these is from these studies: 1, Henry et al. (Henry et al., 2002); 2, this study; 3, Duncan et al., Li and Warren et al. (Duncan et al., 2001; Li, 1997; Warren et al., 2002).

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