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Calcium signalling and cell polarity

Generation of complex spatiotemporal Ca²⁺ signals is critical for numerous physiological processes. Cells achieve this by localizing Ca²⁺ pumps and channels to specific subcellular regions. Inositol 1,4,5-trisphosphate receptors (IP₃Rs) that release Ca²⁺ from the ER, for example, are often concentrated at one pole but this depends on the cell type. So how are these patterns of receptor localization established and maintained? Jean-Pierre Mauger and co-workers have used immunofluoresence confocal microscopy and immunogold EM analysis to probe the relationship between IP₃Rs and polarity in MDCK cells. They observe that, in non-polarized cells, IP₃Rs are present throughout the ER but that as the cells polarize IP₃Rs become concentrated around tight junctions at the apical region of the lateral membrane (see p. 2791). The authors go on to show that culturing cells in Ca²⁺-depleted medium, which disrupts polarity, rapidly abolishes the pattern of IP₃R localization and that this slowly returns following restoration of Ca²⁺, which repolarizes cells. They conclude that receptor localization is intricately linked to cell polarization, suggesting that IP₃Rs are recruited to forming tight junctions, where local Ca²⁺ release could have numerous roles.

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The type 3 inositol 1,4,5-trisphosphate receptor is concentrated at the tight junction level in polarized MDCK cells

Pascal Colosetti, Richard E. A. Tunwell, Caroline Cruttwell, Jean-Pierre Arsanto, Jean-Pierre Mauger, and Doris Cassio
JCS 2003 116: 2791-2803. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?