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Fig. 4. Bni1p controls cell polarity in budding yeast through the assembly of actin cables. Bni1p is activated by Rho GTPases (1). Activated Bni1p nucleates and assembles actin cables (2). Tropomyosin stabilizes the growing cables (3). Bni1p binds to the barbed ends of cables, thereby establishing their polarity. In turn, the polarized cables can then serve as tracks for myosin V, Myo2p, which migrates towards the barbed end of the actin cable to deliver secretory vesicles (4) and to orient the mitotic spindle (5).





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