Fig. 8. Centrin and bb development. (A) Isolated cytoskeletons of N41 stained with anti-centrin (a-c; pCen2) and anti-acetylated tubulin (a'-c'). a''-c'', merged images. Centrin was absent from probasal bodies (arrowheads in a'' and b''), which in some cells occurred in aberrant numbers (b). Arrowhead in c'', additional, nonflagellate bbs containing centrin emphasizing that bbs acquire centrin during their development. (B) Analysis of N41 isolated cytoskeletons with GT335 (a-d) and either monoclonal (BAS6.8; a',b') or polyclonal anti-centrin (pCen2; c',d'). Bar, 5 µm. Note the presence of bbs without or with only faint centrin staining. (C) Centrin content in flagellate and nonflagellate bbs of A7 or A10. Top, bbs stained with anti-centrin; adjustments of contrast and brightness were identical for all images. The rectangle shows the size of the box used for intensity measurements. Arrowheads, flagella; arrow, bald bb. Bottom, fluorescence intensities obtained by anti-centrin staining in arbitrary units. Note the reduced amount of centrin in bbs of A10, especially bald ones. Standard deviations and the number of measurements are indicated.

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