Fig. 5. IL-1 exhibits the ability to displace carboxyfluorescein from the dioleylphosphoglycerol micelles. DOPG liposomes containing CF were prepared as described in Materials and Methods; the micelle was introduced into the cuvette at a 1:60 dilution in 10 mM HEPES, pH 7.0 containing 150 mM NaCl and allowed to equilibrate. At the 120 second time point, the experiment was initiated by introducing either 0.1% Triton X-100 (positive control, ), 0.5 µM of -Chymotrypsin (negative control, —), and different concentrations of IL-1, 0.5 µM (), 0.25 µM (), 0.125 µM () 0.062 µM (x) and 0.031 µM ().

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