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Fig. 4. Changes in the electrophoretic mobility of EDEN-BP are due to its phosphorylation and dephosphorylation. (A) Extracts from non-activated eggs and calcium ionophore-activated eggs were incubated at 30°C with (+) or without (-) phophatase {lambda} for the indicated times. The extracts were analysed on 15% Anderson SDS/PAGE and immunoblotted with anti-EDEN-BP antibodies. (B) EDEN-BP immunoprecipitated from unfertilized egg extract was incubated with phospatase {lambda} and treated as above. The different electrophoretic forms of EDEN-BP are indicated on the left.





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