Pulsatory movements of the apical vesicle accumulation in a Lilium pollen tube during normal growth exhibiting periodic growth rate fluctuations visualised by confocal imaging of FM4-64 (Fig. 1A) (Parton et al., 2001).

The BFA-induced membrane aggregation visualised by confocal imaging of FM4-64 in a Lilium pollen tube (Fig. 1B; Fig. 8B).

Tracking the movements of mitochondria in a growing Lilium pollen tube with Mitofluor staining (Fig. 1E).

Tracking the movements of mitochondria in a BFA-treated pollen tube with Mitofluor staining. Restriction of movement to behind the main BFA aggregation (arrow) (Fig. 1F).

The BFA-induced aggregation visualised by DIC imaging, highlighting the movement of material from the tip and its subsequent fusion (sequence from Fig. 8A).

Effects of heat treatment on the periodic movements associated with the BFA-induced membrane aggregation (Fig. 10C).

Calcium ratio imaging in a BFA-treated Lilium pollen tube. Note the regions of dye exclusion corresponding to the BIA and associated aggregations that tend to give rise to local artefacts in calcium measurement owing to low dye signal. Fluorescence image was contrast enhanced to reveal negative staining of the BIA and associated aggregations (see Fig. 11 for look-up table).