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Fig. 1. BFA effects the organisation of living pollen tube tips. Confocal images of
FM4-64 distribution in L. longiflorum (A,B) and N.
plumbaginifolia (C,D) before and 30-60 minutes after 3.6 µM BFA
treatment. (E-F) Confocal images of mitofluor staining before and after BFA
treatment in L. longiflorum. (G-H) Schematic of cytoplasmic movements
in (G) growing and (H) BFA-treated pollen tube, based upon image sequences.
(I) Projected time series of an FM4-64-stained, BFA-treated tube showing
tracks of movement. Bars, 15 µm (A,B,E,F) or 10 µm (C,D); corresponding
bright field images shown at one-third size.
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