Fig. 6. (A-C) Differences in ER and Golgi involvement in the BIA and associated aggregates revealed by fluorescein protein expression in living L. longiflorum pollen tubes treated with 3.6 µM BFA. (A) mGFP5-ER redistribution (times in minutes). Arrowheads indicate fine structures projecting into the apex in the main picture and contrast adjusted insert. (B-C) Redistribution of Golgi markers GT-EYFP and GONST1-YFP (times after applying BFA in minutes). In all cases, before the 15-minute time-point tubes were still extending so images have been aligned from the tips. Images for time-points 40 minutes and longer are contrast adjusted to increase brightness relative to earlier time points. Arrowheads in C indicate the BIA. Inserts show bright field images at one-third size. Bars, 15 µm.

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