Fig. 5. Dynamic movement of Meu14-GFP in live meiotic cells as revealed by time lapse observations. (A) Cells (YDO120) passing from prometaphase II to metaphase II. (i) Merged images of Meu14-GFP (green) and nuclei stained by Hoechst 33342 (blue) recorded at 2 minute intervals and shown at 6 minute intervals. (ii) An enlarged Meu14-GFP image at 0 minutes is shown to highlight the appearance of the Meu14-GFP dot/ring structure (arrowhead) at the end of the nucleus. (B) Cells passing from metaphase II to anaphase II. (C) Three-dimensional images of the Meu14-GFP structure during anaphase II. (D) Enlarged views of Meu14-GFP localization at the late stage of meiosis II. A thin pouch-like image containing trace amounts of Meu14-GFP is observed, at the end of which a strong band of Meu14-GFP is detected. Bar, 10 µm. Please refer to Movies 1-4 for the dynamic Meu14-GFP profile and Movie 5 for three-dimensional images of Meu14-GFP rings.

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