Fig. 7. Pre-incubation of MT-bound vesicles with GDP results in increased motility and fission. Fluorescent early endocytic vesicles were prepared after injection of a rat with Texas-Red-labeled ASOR. Endocytic vesicles were flowed into a 3 µl microscopy chamber in which Taxol-stabilized rhodamine-labeled MTs had been attached to the glass surface. MT-bound vesicles were incubated for 5 minutes with buffer alone (A) or with buffer containing 4 mM GDP (B). Time in seconds after addition of 50 µM ATP is shown at the bottom of each panel. Arrows point to examples of vesicles bound to MTs. In subsequent panels, smaller arrows indicate motile vesicles while larger arrows point to the original vesicle position (A,B). In B, arrowheads point to vesicles undergoing fission. Bar, 10 µm.

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