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Fig. 5. Localization of Rec8 on chromatin loops in meiotic prophase. Nuclear
spreads from mouse testicular cells were prepared by treatment of cells with
85 mM NaCl (A) or 75 mM KCl (B) before fixation in 1% paraformaldehyde with
SDS, as described in Materials and Methods. The samples were
immunofluorescently stained with anti-Rec8 antibody. DNA was counter-stained
with propidium iodide. (C) Magnification of the region indicated in panel B.
Bar, 2 µm.
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