Fig. 2. Colocalization of GFP-TIVAMP and GFP-cellubrevin but not of GFP-endobrevin with syntaxin 1 in the ER of syntaxin-1-expressing cells. MDCK cells stably expressing GFP-TIVAMP, GFP-cellubrevin (GFP-Cb) or GFP-endobrevin (GFP-Eb) were fixed 24 hours after transfection with syntaxin 1. Cells were stained for syntaxin 1 (red) and calreticulin (blue). Insets show confocal images acquired at high magnification of the same transfected cells shown in the lower magnification panels. GFP-TIVAMP and GFP-Cb lost their characteristic vesicular pattern and instead colocalized with calreticulin in syntaxin-1-expressing cells, as shown by the triple colocalization in the merge panels (light pink). GFP-Eb pattern was not affected by syntaxin 1 expression, as indicated by the colocalization of syntaxin 1 and calreticulin in syntaxin-1-expressing MDCK cells, but not of GFP-Eb, in the merge panel (magenta/purple). Bar in low magnification panels, 7 µm; bar in high magnification insets, 4 µm.

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