Fig. 7. TI-VAMP relocalization in syntaxin-1-expressing cells was microtubule dependent. (A) MDCK cells stably expressing GFP-TIVAMP incubated with or without 5 µM nocodazole for 1 hour were fixed and stained for -tubulin. Note that in the presence of nocodazol, microtubules were completely disrupted. (B) MDCK cells stably expressing GFP-TIVAMP incubated with or without 5 µM nocodazole for 1 hour prior to transfection with syntaxin 1 were fixed and stained for syntaxin 1 8 hours after transfection. In control conditions (Cont), the expression of syntaxin 1 and its retention in the ER induced a relocalization of GFP-TIVAMP towards syntaxin-1-positives structures in the ER (arrows). By contrast, in the absence of functional microtubules (+Noc) the localization of GFP-TIVAMP in transfected cells was indistinguishable from non-transfected cells. Bar, 4 µm.

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