Phagosomal oxidative activity during ß2 integrin (CR3)-mediated phagocytosis by neutrophils is triggered by a non-restricted Ca2+ signal: Ca2+ controls time not space
J Cell Sci
Dewitt et al. 116 (14): 2857.
JCS00499 Movie
Files in this Data Supplement:
Movie 1
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The
animated sequence shows the complete data for the fluorescent images shown in
Fig. 4a. On the right is the fura2 ratio image from the neutrophil (Fig 4a,
middle row) combined with the DCDHF intensity from the C3bi-opsonised zymosan
particle (Fig 4a, bottom row). The sequence begins on contact of the particle
with the neutrophil and continues until the particle is completely
internalised. On the left is the read out of the particle-associated DCDCF
signal updated at 1-2 second intervals during internalisation. The complete
time course for the Ca2+ signal is superimposed on the oxidative
response at the end of the sequence for clarity.
