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Fig. 4. Crumbs 3 overexpression leads to a disruption of apico-basal polarity in MDCK cysts cultured in collagen matrix. (A) Wild-type MDCK cells were cultured in collagen gel according to Materials and Methods to induce three-dimensional cyst formation. Cysts were co-stained with the indicated antibodies. (B,C) MDCK cells expressing the various Myc-CRB3 proteins were cultured in collagen and subsequently immunostained using the indicated antibodies.





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