Fig. 6. Effects of Myc-Lin-2/Pals1 PDZ chimera expression on junctional assembly and apico-basal polarity in MDCK monolayers. (A-B) MDCK stable cell lines expressing either Myc-Lin-2/Pals1 PDZ or Myc-Lin-2PDZ (negative control) were subjected to a calcium switch experiment as in Fig. 3. At the indicated timepoints, cells were co-immunostained with anti-ZO-1 and anti-E-cadherin antibodies (green and red, respectively). (C) MDCK cells expressing the indicated proteins were grown in low calcium media (t=0) or in normal calcium media for 24 hours. Subsequently, they were co-stained with antibodies directed against E-cadherin (red) and either gp135 or CRB3 as indicated in green.

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