Fig. 6. Differences in fibroblast migration to TSP1 and hep I. (A) Wild-type and CRT-knockout (KO) MEF migration in response to serum-free media, with or without hep I (100 nM), TSP1 (7.8 nM) or FBS (0.1%), was assessed as previously described. Average cell migration speed under each condition was determined by dividing total migration distance by the total time of the assay. At least 75 cells were analyzed for each condition. n=3-4, *P<0.05, **P<0.01. (B) Percent of cells migrating under each condition was determined by dividing the number of cells migrating at least 0.06 microns/minute by the total number of cells. At least 75 cells were analyzed for each condition. n=3-4, *P<0.05, **P<0.01. (C) Total cellular displacement was determined as the difference between final cell position and initial cell position. At least 75 cells were analyzed for each condition. n=3-4, *P<0.05, **P<0.01, ***P<0.001. (D) Directionality of cell migration was determined by dividing the total cellular displacement by the total distance of migration. Under this method, a purely directional response will show a ratio of 1, whereas increasingly random migration responses will show ratios approaching zero. At least 75 cells were analyzed for each condition. n=3-4, *P<0.05, **P<0.01, ***P<0.001.

Return to article