Fig. 8. RAP pretreatment blocks TSP1/hep I-induced endothelial cell migration. BAE cells were plated at low density under serum-free conditions onto glass coverslips coated with vitronectin and fibronectin. Cells were allowed to attach for 3 hours. Some coverslips were pretreated for 30 minutes with the LRP inhibitor receptor-associated protein (RAP) at 50 nM. Coverslips were loaded onto the Dunn Chamber in serum-free media (A,C,E,G) or serum-free media containing RAP (B,D,F,H), as well as 100 nM hep I (C,D), 7.8 nM TSP1 (E,F) or 0.1% FBS (G,H). The chamber was sealed, and time-lapse video was taken of the cells over a 7-hour time span. Migration of individual cells was tracked using Metamorph software and individual tracks were analyzed for distance, displacement, directionality and orientation. Final positions of the cells are plotted with the initial point being the origin. Results are representative of at least three separate experiments. Average displacement () is given for each treatment. *P<0.05, **P<0.01.

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